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The hormone levels in plasma and brain were determined by spectrophotofluorometry and radioimmunoassay kit. 放射免疫法及熒光分光光度法檢測血漿和腦組織激素水平。
Methods:Animal diabetics models were made with ip alloxan. Blood glucose was measured with glucose oxidase method(kit), and insulin was measured with radioimmunoassay(kit). 方法:採用四氧嘧啶誘發實騐性糖尿病動物模型,用葡萄糖氧化酶法測血糖,用放射免疫法測血清胰島素。
Methods: Plasma ET levels were determined by using a radioimmunoassay kit, serum NO levels were determined by using a nitrate reductase method in 20 newborn with HIE and 20 normal newborn. 方法: 採用放射免疫分析法和硝酸還原酶法測定20 例HIE新生兒和20 例正常新生兒血漿ET與血清NO水平。