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At present, little researches have been carried out about the pig skeletal muscle satellite cells. 本研究中,我们建立并完善了猪骨骼肌卫星细胞的培养方法。
Rat triceps brachii muscle was acquired to separate skeletal muscle satellite cells with the two-step method of collegenase-1 and trypsin and were cultured and subcultured in vitro. 取大鼠肱三头肌,采用二步消化法分离骨骼肌卫星细胞,体外进行原代培养和传代培养。
OBJECTIVE:To observe the light electron microscopic structure and the immunohistochemical characters of the skeletal muscle satellite cells in vitro in the myogenesis of the neonatal mice. 目的:观察体外培养的小鼠骨骼肌卫星细胞在成肌过程中的光电镜结构及免疫组织化学特性。